Description
A single-step rapid screening test for semi-quantitative determination of alcohol in saliva.
Approved for forensic use.
The Saliva Alcohol Rapid Test Strip is a rapid, highly sensitive method to detect the presence of alcohol in saliva and provide an approximate value of relative blood alcohol concentration.
This test only provides a preliminary screening. To obtain confirmation of the analytical result, a more specific alternative chemical method must be used. Clinical evaluation and professional opinion should be applied to the result of any test, especially when preliminary investigations are positive.
It has been found that the concentration of alcohol in saliva is comparable to that in blood. The Saliva Alcohol Rapid Test Strip consists of a plastic reaction strip with a reactive pad positioned at the end. In contact with alcohol solutions, the reactive pad will immediately change color, depending on the concentration of alcohol present in the blood. The reactive pad employs a solid-phase chemical system that utilizes a highly specific enzymatic reaction.

How to use
Instructions for use
Before testing, allow the test strip in the package to reach room temperature (15-30 °C).
1. Avoid putting anything in your mouth for fifteen minutes before the start of the test. This includes non-alcoholic beverages, tobacco products, coffee, breath mints, food, etc.
2. Spit saliva into the collection container.
3. Open the foil package and remove the test strip. Observe the reactive pad at the end of the strip. If, before applying the saliva sample, the reactive pad is blue, do not use it.
4. Saturate the reactive pad with saliva from the collection container or by applying saliva directly to the pad. (Saturation usually takes 6-8 seconds). Immediately after applying the saliva, start the timer.
5. Read the result after two minutes. Compare the color of the reactive pad with the color chart on the foil package to determine the relative blood alcohol concentration.

Interpretation of results
Positive: The Saliva Alcohol Rapid Test Strip will change color in the presence of alcohol in the saliva. The color can range from light blue with a relative blood alcohol concentration of 0.02% up to dark blue with a relative blood alcohol concentration near 0.30%. This range of pad colors allows for an approximate value of the relative blood alcohol concentration to be obtained. The test may produce colors that appear to be between two adjacent colored pads.
NOTE: The Saliva Alcohol Rapid Test Strip is very sensitive to the presence of alcohol. A lighter blue color than the colored pad corresponding to 0.02% should be interpreted as positive for the presence of alcohol in the saliva but with a relative blood alcohol concentration lower than 0.02%.
Negative: If the Saliva Alcohol Rapid Test Strip shows no color change, this result should be interpreted as negative, indicating that no alcohol was detected.
Invalid: If, before applying the saliva sample, the reactive pad is blue, do not use it.
NOTE: If the outer edges of the colored pad produce a slight coloration, but most of the pad remains colorless, the test should be repeated to ensure complete saturation of the pad with saliva. The test cannot be reused.

Precautions
The Saliva Alcohol Rapid Test Strip is a test that allows for visual interpretation, using color matching to provide an approximate value of the relative blood alcohol concentration. Test materials that have been exposed to saliva should be treated as potentially infectious. Do not use the Saliva Alcohol Rapid Test Strip after the expiration date indicated on the foil package.

Restrictions
1. If 15 minutes are not waited before performing the test after eating, drinking, or smoking, incorrect results may be obtained due to possible contamination of saliva with interfering substances.
2. The Saliva Alcohol Rapid Test Strip is very sensitive to the presence of alcohol. Alcohol vapors in the air can sometimes be detected by the Saliva Alcohol Rapid Test Strip. Alcohol vapors are present in many public buildings and homes. Alcohol is a component of many household products such as disinfectants, deodorants, perfumes, and glass cleaners. If alcohol vapors are presumed to be present in the environment, it is recommended to perform the test in a vapor-free area.
3. Ingestion or use of over-the-counter medications and products containing alcohol may produce positive results.

Storage
The Saliva Alcohol Rapid Test Strip should be stored at 2-30 °C (36-86 °F) in its sealed foil package. If the storage temperature is above 30 °C, the test performance may be altered. If the product is refrigerated, the Saliva Alcohol Rapid Test Strip must be brought to room temperature before opening the package.

Format
Test strips, collection container, leaflet.